STANDARD ANALYSES PERFORMED INCLUDE

• pH

• Concentration (absorbance)

• Conductivity

pH

The requirement for pH differs between solutions used during production and end product. Please see the CoA for information about a specific product. pH requirements for a final product may be requested by customer.

Protein concentration measurement

Protein concentration measurements of IgY and affinity purified antibodies is required since this is the determination of amount of antibody provided to the customer. Protein concentration is determined by using a spectrophotometer to measure the optical density (OD) of the final product. Protein concentration of complete IgY will be adjusted to 18 to 23 mg/ml. Protein concentration of affinity purified antibodies is usually in the range from 1.0 to 3.0 mg/ml.

Conductivity measurement

There are set requirements for the conductivity of both complete IgY and affinity purified IgY antibody. Conductivity is measured in IgY after dialysis and requirements depend on the buffer conditions. The same requirements apply to the conductivity of the finished affinity purified antibody products.